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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been observed in several tumors, which leads to chromosome instability, and so may possibly add to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant function in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be described. Two additional splice variants ended up described but in depth nucleotide sequence info just isn't nevertheless offered.

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Blocking peptides are peptides that bind specially for the focus on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged via the antibody.

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This guidebook outlines even further feasible optimization of mobile and immunoassay parameters to make certain the very best benefits are...

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This information outlines additional probable optimization of cellular and immunoassay parameters to guarantee the best possible effects are...

Antibodies bound to the blocking peptide not bind into the epitope around the focus on protein. By evaluating the staining from the blocked antibody compared to the antibody on your own, you can see which staining is distinct.

Focus on Facts Cyclin E belongs for the remarkably conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary which is degraded as cells progress by means of S phase.

The measurement of get more info protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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